Concanavalin a type iv

Descriptions

Analysis Note

Where reported, agglutination activity is expressed in µg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, containing Ca²⁺ and Mn²⁺ of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C.

Application

Concanavalin A from Canavalia ensiformis (Jack bean) has been used:as T-lymphocyte mitogen in lymphocyte proliferation assay for correlations with organochlorine contaminants (OCs)for in vitro stimulation of T cell hybridomas, BW5147 cells and primary CD4⁺ T cells for T cell vaccination and for induction of hepatitisfor the stimulation of mice splenocytes in the suppression of T-lymphocytes proliferation assayfor the stimulation of spleen in mitogen assays

Biochem/physiol Actions

Con A is not blood group specific but has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. Ca²⁺ and Mn²⁺ ions are required for activity. Con A dissociates into dimers at pH 5.6 or below. Between pH 5.8 and pH 7.0, Con A exists as a tetramer; above pH 7.0 higher aggregates are formed. Con A exhibits mitogenic activity which is dependent on its degree of aggregation. Succinylation results in an active dimeric form which remains a dimer above pH 5.6.

General description

Concanavalin A(con A) is a plant lectin refined from jack beans. It is a tetrameric protein, which has four identical 28 kDa subunits. Con A has the ability to connect with the mannose residues of several glycoproteins and can stimulate lymphocytes.

Packaging

1 g in glass bottle

100, 250 mg in glass bottle

25 mg in poly bottle

Quality

Highly purified by specific affinity chromatography

Specifications

No detailed specifications are available for this product.

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Code	Description	Quality Level	Manufacturer	Storage Temp.	Impurities	Form	Price	Quantity
3586667	Concanavalin a type iv	300	SIGMA-ALDRICH	−20°C	salt, essentially free, Carbohydrate, essentially free	lyophilized powder	£105.02 (exc VAT) per 100MG	- +
3586669	Concanavalin a type iv	300	SIGMA-ALDRICH	−20°C	salt, essentially free, Carbohydrate, essentially free	lyophilized powder	£218.68 (exc VAT) per 250MG	- +
3586670	Concanavalin a type iv	300	SIGMA-ALDRICH	−20°C	salt, essentially free, Carbohydrate, essentially free	lyophilized powder	£42.62 (exc VAT) per 25MG	- +