?-Amylase from sweet potato has been used:as a molecular weight marker in blue native polyacrylamide gel electrophoresis (PAGE)as a standard for calibration curve preparation in size exclusion chromatographyas a model protein for small-angle X-ray analysis (SAXs)
Biochem/physiol Actions
?-Amylase acts on the polysaccharide ?(1,4) glycosidic linkages and releases maltose present in the non-reducing ends. Due to its stability, ?-Amylase from sweet potato is used in the immobilized enzyme preparation in combination with agarose beads.
General description
?-Amylase from sweet potato is a tetrameric enzyme with each monomer comprising (?/?) core domain and a C-terminal loop region. It belongs to the glycoside hydrolase family GH14.