Phospholipase C, Phosphatidylinositol-specific from Bacillus cereus, buffered aqueous glycerol solut
Analysis Note
Acetylcholinesterase is measured according to Ellman, et al.
Application
Phospholipase C, Phosphatidylinositol-specific from Bacillus cereus has been used:in the hydrolysis of substrates p-nitrophenylphosphorylcholine (p-NPPC) and p-nitrophenylphosphorylphosphate (p-NPP)to cleave glycosylphosphatidylinositol (GPI) anchor of lynx1 protein and its detachment from plasma membraneto cleave immunolabeled HeLa cells
Biochem/physiol Actions
Phospholipase C (PI-PLC) releases diacylglycerol by cleaving the glycosylphosphatidylinositol. It has broad substrate specificity and its activity is influenced by metal ions and surfactants.
Used for the release of GPI anchored proteins from the membrane.
General description
Phospholipase C (PI-PLC) from Bacillus cereus has irregular triosephosphate isomerase (TIM)-barrel structure with eight-standard parallel ? barrel. It is a 28 kDa protein.
Packaging
5, 25 units in poly bottle
Physical form
Solution in 60% (v/v) glycerol containing 10 mM Tris-HCl, pH 8.0 and 10 mM EDTA
Unit Definition
One unit will liberate one unit of acetylcholinesterase per minute from a membrane-bound crude preparation at pH 7.4 at 30 °C (10 minute incubation).
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