Concanavalin A from Canavalia ensiformis (Jack bean), agarose conjugate, Type VI, saline suspension

Concanavalin A from Canavalia ensiformis (Jack bean), agarose conjugate, Type VI, saline suspension

Analysis Note

Where reported, agglutination activity is expressed in µg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, containing Ca2+ and Mn2+ of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C.

Application

Concanavalin A (Con A) has been used for enzyme immobilization.

Used for the purification of glycoproteins containing mannose

Biochem/physiol Actions

Concanavalin A (Con A) is a T cell mitogen that can activate the lymphocytes.Con A can induce tumor cell death through autophagy.

Con A is not blood group specific but has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. Ca2+ and Mn2+ ions are required for activity. Con A dissociates into dimers at pH 5.6 or below. Between pH 5.8 and pH 7.0, Con A exists as a tetramer; above pH 7.0 higher aggregates are formed. Con A exhibits mitogenic activity which is dependent on its degree of aggregation. Succinylation results in an active dimeric form which remains a dimer above pH 5.6.

General description

Concanavalin A (Con A), isolated from Jack beans (Canavalia ensiformis), is a mannose or glucose specific-binding lectin. The lectin is a tetramer and each subunit possesses a saccharide-binding site. Con A can be isolated by affinity chromatography with dextrans.

Physical form

Suspension in 1.0 M NaCl + 0.1 M potassium phosphate, pH 6.0, with 1 mM CaCl2, 1 mM MgCl2 and 1 mM MnCl2 containing preservative

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Code Description Capacity Manufacturer Form Price Quantity
3586699 Concanavalin A from Canavalia ensiformis (Jack bean), agarose conjugate, Type VI, saline suspension 5-15 mg/mL binding capacity (yeast mannan) SIGMA-ALDRICH saline suspension
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3586700 Concanavalin A from Canavalia ensiformis (Jack bean), agarose conjugate, Type VI, saline suspension 5-15 mg/mL binding capacity (yeast mannan) SIGMA-ALDRICH saline suspension
£653.25 (exc VAT) per 25ML
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3586701 Concanavalin A from Canavalia ensiformis (Jack bean), agarose conjugate, Type VI, saline suspension 5-15 mg/mL binding capacity (yeast mannan) SIGMA-ALDRICH saline suspension
£193.61 (exc VAT) per 5ML
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