Lectin from Helix pomatia, lyophilized powder, salt, free

Descriptions

Analysis Note

Where reported, agglutination activity is expressed in µg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of human blood group A erythrocytes after 1 hour incubation at 25 °C.

Application

Lectin from Helix pomatia has been used to conjugate with biotin and acridinium and used in lectin-agglutination tests.

Biochem/physiol Actions

HPA has anti-A human blood group specificity and has an affinity for terminal N-acetyl-α-D-galactosaminyl residues. Conjugates are prepared from affinity purified lectin.

Lectin from Helix pomatia protects fertilized eggs by coating them with perivitelline fluid. They exhibit antibacterial activities and immunity in snail. Helix pomatia agglutinin (HPA) possess the potential to agglutinate pathogens. HPA has also emerged as a marker of altered Oglycosylation in cancer.

General description

Lectin from Helix pomatia is a hexameric glycoprotein with a molecular mass of 79 kDa. It issecreted by the albumen gland of the roman snail. Helix pomatia agglutinin (HPA) is specific for N-acetylgalactosamine (GalNAc).

Packaging

1, 5 mg in glass bottle

Preparation Note

Affinity purified by a modification of the method of Hammarstrom and Kabat.

Specifications

No detailed specifications are available for this product.

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Code	Description	Quality Level	Manufacturer	Storage Temp.	Impurities	Form	Price		Quantity
3587065	Lectin from Helix pomatia, lyophilized powder, salt, free	200	SIGMA-ALDRICH	−20°C	salt, free	lyophilized powder	£188.04 (exc VAT) per 1MG		- +
3587066	Lectin from Helix pomatia, lyophilized powder, salt, free	200	SIGMA-ALDRICH	−20°C	salt, free	lyophilized powder	£642.11 (exc VAT) per 5MG		- +