Atto 565 NHS ester, BioReagent, suitable for fluorescence, >=70.0% (coupling to amines)
Features and Benefits
Strong absorption High fluorescence quantum yield High thermal and photo-stability
General description
Atto 565 NHS ester is a commonly used amine-reactive reagent, provided by ATTO-TEC. It is widely used in protein labeling and readily reacts with compounds containing amino groups, forming a chemically stable amide bond between the dye, e.g., a protein. The optimum pH-range for NHS-ester coupling is pH 8.0 – 9.0. At this pH amino groups of proteins, i.e., the ε-amino groups of lysines are unprotonated to a degree sufficiently high for fast coupling.
Legal Information
This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.
Other Notes
Fluorescence of bioconjugates; employed in fluorescence resonance energy transfer, FRET, experiments.
| Quality Level | 100 |
|---|---|
| Manufacturer | SUPELCO |
| Product Line | BioReagent |
| Storage Temp. | −20°C |
| Suitability | suitable for fluorescence |
| Form | powder |
| Assay | ≥90% (degree of coupling), ≥90% (HPLC) |
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