GenElute#-E Blood DNA High Yield Kit

GenElute#-E Blood DNA High Yield Kit

Application

Traditional methods for purifying genomic DNA from liquid blood samples ≥ 200 µl rely on medium and large format silica (bind-wash-elute) purification kits, which suffer from limited binding capacity, low DNA concentration, and low purity. The GenElute™-E Single Spin Blood DNA High Yield Kit, extracts up to 20 µg highly concentrated DNA (200 ng/µl) while offering the flexibility of sample inputs ranging from 200 µl to 1 ml liquid blood. EDTA-, citrate- as well as heparin-stabilized human and animal blood can be used. Enriched DNA yield and concentration make this kit well suited for researchers with limited or low-titer samples who require high sensitivity for NGS or other detection methods. Purified genomic DNA is eluted in Tris buffer, pH 7.8 and can immediately be used for most common downstream applications, including PCR, genotyping, NGS, and others. Genomic DNA preparations usually show an A260/280 ratio of 1.8 to 2.0.

Store enzymes at 2-8°C for up to 12 months. All other components should be stored at room temperature.

Features and Benefits

Fast and efficient purification: 10-minute lysis and minimal hands-on, preparation stepsHigher quality DNA: negative chromotography-based purification reduces carryover of PCR inhibitors and other contaminents Reduced environmental impact: 55% less plastic waste, as well as a significant reduction in hazardous chemicals

General description

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is aligned with "Waste prevention" and "Designing safer chemicals". Click here for more information.

GenElute™-E purification kits provide a simple alternative for DNA purification. Compared with traditional methods, GenElute™-E offers: Isolation of DNA in under an hour with minimal sample handling Better purity, leading to improved performance in PCR and other applications Significant reduction in plastic and hazardous chemical wasteTraditional silica-based, bind-wash-elute purification kits require multiple wash steps to remove impurities from the spin columns. These steps increase the risk of cross contamination, subject the DNA to centrifugation sheering forces, and introduce chaotropic salts that can carry over into the final sample, inhibiting downstream applications.GenElute™-E kits employ size exclusion?negative chromatography to separate large nucleic acid molecules from smaller protein, lipid, and ionic components in cell, tissue, blood, and other samples. Single-spin columns efficiently absorb and retain cellular debris and sample contaminants while allowing nucleic acids to pass through, reducing the number of steps and?plastic?materials required for purification.?This novel method for high-quality purification is made possible by our innovative SmartLyse® Protease, which enables fast and efficient lysis of a wide range of sample types.Isolate nucleic acid in a fraction of time compared to traditional silica bind-wash-elute procedures. Simply mix sample with lysis components and incubate according to kit protocol, pipette sample onto the spin column, and centrifuge directly into a collection tube

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Code Description Manufacturer Feature Price Quantity
3586849 GenElute#-E Blood DNA High Yield Kit SIGMA-ALDRICH Compatible Application (Suitable for most common downstream applications, including genotyping, PCR, and NGS), Typical/expected yield (Varies by sample. Please
£70.48 (exc VAT) per EACH
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3586850 GenElute#-E Blood DNA High Yield Kit SIGMA-ALDRICH Compatible Application (Suitable for most common downstream applications, including genotyping, PCR, and NGS), Typical/expected yield (Varies by sample. Please
£1,161.64 (exc VAT) per EACH
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3586851 GenElute#-E Blood DNA High Yield Kit SIGMA-ALDRICH Compatible Application (Suitable for most common downstream applications, including genotyping, PCR, and NGS), Typical/expected yield (Varies by sample. Please
£257.68 (exc VAT) per EACH
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