Lectin from Phaseolus vulgaris (red kidney bean), Phytohemagglutinin PHA-M, lyophilized powder, BioR

Lectin from Phaseolus vulgaris (red kidney bean), Phytohemagglutinin PHA-M, lyophilized powder, BioR

Analysis Note

Agglutination activity is expressed in µg/mL and is determined from serial dilutions of a 1 mg/mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes in phosphate buffered saline, pH 6.8, after 1 hr incubation at 25 °C.

Application

Lectin from Phaseolus vulgaris (red kidney bean) is used in Lymphocyte Transformation Assay and culture medium.

Biochem/physiol Actions

PHA consists of two molecular species, an erythroagglutinin (PHA-E) which has low mitogenic activity and high erythroagglutinin activity, and leucoagglutinin (PHA-L) which has high mitogenic and leucoagglutinating activity, but very low erythroagglutinating activity. PHA-E is not blood group specific, but agglutination can be inhibited by certain oligosaccharides. PHA-P is the protein form of PHA prior to separation and purification of erythroagglutinin and leucoagglutinin. PHA-M is the mucoprotein form. Conjugates are prepared from the corresponding purified lectins.

Packaging

25, 100 mg in glass bottle

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Code Description Quality Level Manufacturer Technique(s) Product Line Storage Temp. Impurities Solubility Form Price Quantity
3587113 Lectin from Phaseolus vulgaris (red kidney bean), Phytohemagglutinin PHA-M, lyophilized powder, BioR 200 SIGMA-ALDRICH cell culture - mammalian: suitable BioReagent 2-8°C salt, free PBS: 2 mg/mL lyophilized powder
£619.82 (exc VAT) per 100MG
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3587114 Lectin from Phaseolus vulgaris (red kidney bean), Phytohemagglutinin PHA-M, lyophilized powder, BioR 200 SIGMA-ALDRICH cell culture - mammalian: suitable BioReagent 2-8°C salt, free PBS: 2 mg/mL lyophilized powder
£160.18 (exc VAT) per 25MG
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3587115 Lectin from Phaseolus vulgaris (red kidney bean), Phytohemagglutinin PHA-M, lyophilized powder, BioR 200 SIGMA-ALDRICH cell culture - mammalian: suitable BioReagent 2-8°C salt, free PBS: 2 mg/mL lyophilized powder
£62.12 (exc VAT) per 5MG
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