PNGase F from Elizabethkingia miricola, buffered aqueous solution

Descriptions

Application

Used to deglycosylate protein.

PNGase F from Elizabethkingia miricola has been used for the deglycosylation of the proteins.

Biochem/physiol Actions

Cleaves an entire glycan from a glycoprotein provided the glycosylated asparagine moiety is substituted on its amino and carboxyl terminus with a polypeptide chain.

General description

PNGase F (Peptide N-glycosidase F) cleaves asparagine-linked glycoproteins, which produces carbohydrate-free peptides and detached full-length oligosaccharides.

Physical form

Supplied as a solution in 20 mM Tris HCl, pH 7.5, 50 mM NaCl and 1 mM EDTA

Unit Definition

One unit will catalyze the release of N-linked oligosaccharides from 1 nanomole of denatured ribonuclease B in one minute at 37°C at pH 7.5 monitored by SDS-PAGE. One Sigma unit of PNGase F activity is equal to 1 IUB milliunit.

Specifications

No detailed specifications are available for this product.

Downloads

There are no downloads for this product.

Code	Description	Quality Level	Manufacturer	Storage Temp.	Form	Price		Quantity
3586987	PNGase F from Elizabethkingia miricola, buffered aqueous solution	200	SIGMA-ALDRICH	2-8°C	buffered aqueous solution	£682.50 (exc VAT) per EACH		- +
3586988	PNGase F from Elizabethkingia miricola, buffered aqueous solution	200	SIGMA-ALDRICH	2-8°C	buffered aqueous solution	£398.36 (exc VAT) per EACH		- +