Trypan Blue, powder, BioReagent, suitable for cell culture

Trypan Blue, powder, BioReagent, suitable for cell culture

Stock Code: 3572706
Manufacturer Part No: T6146-100G
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£185.93 (exc VAT) per 100G
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Application


Trypan blue has been used:in cell viability assayto determine the number of living and necrotic cells by staining with 0.2% trypan blue solution.to stain plant tissue in order to to visualize cell death


Biochem/physiol Actions


The trypan blue dye is useful to differentiate the dead and live cells based on the cell?s ability to take up or exclude the dye. A viable cell is assumed to possess an intact cell membrane and hence excludes this dye and the cytoplasm appears clear. While on the other hand non-viable cells shows a blue cytoplasm.


General description


The trypan blue dye is an azo dye, derived from toluidine. It is soluble in water and has a molecular weight of 961 g/mol.


Packaging


5, 25, 100 g in glass bottle

Pack100G
mp>300 °C (lit.)
InChI keyGLNADSQYFUSGOU-UHFFFAOYSA-J
InChI1S/C34H28N6O14S4.4Na/c1-15-7-17(3-5-25(15)37-39-31-27(57(49,50)51)11-19-9-21(55(43,44)45)13-23(35)29(19)33(31)41)18-4-6-26(16(2)8-18)38-40-32-28(58(52,53)54)12
ManufacturerSIGMA-ALDRICH
CompositionDye content, ~40%
Formpowder

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