Sodium bicarbonate has been used:as a component of bovine embryo culture system during in vitro maturation, fertilization, and culture (IVMFC)as a component of a 1:1 mixture of Dulbecco?s modified Eagle?s medium and Ham?s nutrient mixture F-12 during the culture of bovine microvascular endothelial cellsas a component of ITS (insulin transferrin and selenite) medium used during the isolation of dopamine neurons from human embryonic stem cellsas a component of medium used for cell culture of human embryonic kidney cellsas a component of Beltsville thawing solution used for the dilution of semen
General description
Sodium bicarbonate is broadly used as a physiological buffer in cell culture applications. The pKa for bicarbonate ﹤-> carbonic acid reaction is 6.4 The pKa for bicarbonate ﹤-> carbonate reaction is 10.3. Sodum bicarbonate has two pKas, 6.4 and 10.3. It is broadly used as an physiological buffer in in vitro applications.