Bovine Serum Albumin, lyophilized powder, BioReagent, suitable for cell culture, >=96% (agarose gel

Descriptions

Application

Bovine Serum Albumin has been used in the washing solution for flow cytometry analysis, tissue digestion solution and blocking solution for ELISA (enzyme-linked immunosorbent assay). It has also been used in the resuspension buffer for flow cytometry.

Select the right albumin for your application with the Albumin Selection Guide

Biochem/physiol Actions

Bovine serum albumin is broadly used as an additive to cell culture media, especially serum-free media. It provides a range of benefits including protection from oxidative damage and stabilization of other media components such as fatty acids and pyridoxal.

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

General description

Bovine serum albumin is a model protein in various studies and is made up of 583 amino acids. It is a water-soluble protein with a molecular weight of 66.4kDa. Six ?-helices form three homologous domains of BSA. Depending on pH, it undergoes reversible conformational isomerization. The native structure of the protein becomes reactive and flexible on heating.

Packaging

5, 10, 50, 100, 500 g in poly bottle

Preparation Note

Often referred to as Cohn fraction V; this product is prepared by a modified method of the Cohn cold ethanol fractionation method.

Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

Specifications

No detailed specifications are available for this product.

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Code	Description	Quality Level	Pack	pH	Manufacturer	Product Line	Form	Loss	Price	Quantity
3571354	Bovine Serum Albumin, lyophilized powder, BioReagent, suitable for cell culture, >=96% (agarose gel	200	100G	5.0-5.6	SIGMA-ALDRICH	BioReagent	lyophilized powder	≤5%	£894.54 (exc VAT) per 100G	- +
3571355	Bovine Serum Albumin, lyophilized powder, BioReagent, suitable for cell culture, >=96% (agarose gel	200	500G	5.0-5.6	SIGMA-ALDRICH	BioReagent	lyophilized powder	≤5%	Click Add to Quote for price and delivery	- +
3571356	Bovine Serum Albumin, lyophilized powder, BioReagent, suitable for cell culture, >=96% (agarose gel	200	50G	5.0-5.6	SIGMA-ALDRICH	BioReagent	lyophilized powder	≤5%	£603.93 (exc VAT) per 50G	- +
3571357	Bovine Serum Albumin, lyophilized powder, BioReagent, suitable for cell culture, >=96% (agarose gel	200	5G	5.0-5.6	SIGMA-ALDRICH	BioReagent	lyophilized powder	≤5%	Click Add to Quote for price and delivery	- +