M2 medium, With HEPES, without penicillin and streptomycin, liquid, sterile-filtered, suitable for m

M2 medium, With HEPES, without penicillin and streptomycin, liquid, sterile-filtered, suitable for m

Analysis Note

This product is tested for its ability to support the development of one-cell mouse embryos to expanded blastocysts. Minimum requirement is 80% development to blastocyst.

Application

Recommended for manipulation of mouse embryos at ambient temperature. M2 and M16 Medium are common media for in vitro culture of preimplantation stage embryos. It is a modified Krebs-Ringer bicarbonate solution, which is very similar to Whitten′s Medium. M16 contains pyruvate and lactate as energy sources since preimplantation embryos do not utilize glucose efficiently.M2 Medium is a further modification of M16 that substitutes HEPES buffer in place of some of the bicarbonate. M2 is used for collecting and handling embryos for prolonged periods outside a CO2 incubator.

Quality

Presence of particulates in solution will not affect product′s performance.

Reconstitution

Supplement with 0.06 g/L potassium penicillin-G and 0.05 g/L streptomycin sulfate.

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Code Description Quality Level Pack Manufacturer Storage Temp. Impurities Form Price Quantity
3571976 M2 medium, With HEPES, without penicillin and streptomycin, liquid, sterile-filtered, suitable for m 400 100ML SIGMA-ALDRICH 2-8°C endotoxin, tested liquid
£80.62 (exc VAT) per 100ML
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3571977 M2 medium, With HEPES, without penicillin and streptomycin, liquid, sterile-filtered, suitable for m 400 50ML SIGMA-ALDRICH 2-8°C endotoxin, tested liquid
£49.08 (exc VAT) per 50ML
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