Mineral oil, light oil (neat), BioReagent, suitable for mouse embryo cell culture

Mineral oil, light oil (neat), BioReagent, suitable for mouse embryo cell culture

Analysis Note

This material is tested as an overlay in microdrop embryo cultures. (All embryo tested materials are tested using mouse embryos.) Typically BCF1 x BDF1 hybrids are used. The oil is primarily used to prevent evaporation of the medium, particiularly when performing embryo manipulations.1 The procedure is as follows: 1-cell (BCF1 X BDF1) embryos were cultured in triplicate microdrops of embryo-tested HTF (Human Tubal Fluid) medium supplemented with 0.4% BSA. For a valid assay, at least 70% of 1-cell stage control embryos must develop to the blastocyst stage within 96 hours. For product release, at least 80% of 1-cell stage test embryos must develop to the blastocyst stage within 96 hours with the tested material.

Application

Mineral oil has been used as an overlay in microdrop embryo cultures.

Caution

This product is not sterile. Mineral oil is difficult to sterilize. It can only be sterilized by careful filtration of the warmed oil. It is assumed antibiotics will be added to the cell culture medium. Otherwise, Prod. No. M5310 should be used, as it is sterile filtered.

Packaging

1 L in glass bottle

100, 500 mL in glass bottle

5 mL in poly bottle

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Code Description Quality Level Pack InChI key Manufacturer Density Form Price Quantity
3571985 Mineral oil, light oil (neat), BioReagent, suitable for mouse embryo cell culture 200 100ML AEOVEGJBKQQFOP-DDVLFWKVSA-L SIGMA-ALDRICH 0.84 g/mL at 25 °C (lit.) light oil
£40.31 (exc VAT) per 100ML
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3571986 Mineral oil, light oil (neat), BioReagent, suitable for mouse embryo cell culture 200 1L AEOVEGJBKQQFOP-DDVLFWKVSA-L SIGMA-ALDRICH 0.84 g/mL at 25 °C (lit.) light oil
£99.80 (exc VAT) per 1L
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3571987 Mineral oil, light oil (neat), BioReagent, suitable for mouse embryo cell culture 200 500ML AEOVEGJBKQQFOP-DDVLFWKVSA-L SIGMA-ALDRICH 0.84 g/mL at 25 °C (lit.) light oil
£57.86 (exc VAT) per 500ML
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3571988 Mineral oil, light oil (neat), BioReagent, suitable for mouse embryo cell culture 200 5ML AEOVEGJBKQQFOP-DDVLFWKVSA-L SIGMA-ALDRICH 0.84 g/mL at 25 °C (lit.) light oil
£38.51 (exc VAT) per 5ML
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