Superoxide Dismutase from bovine erythrocytes, BioReagent, >=3,000 units/mg protein, suitable for ce

Descriptions

Analysis Note

For assay method, see McCord, J.M. and Fridovich,I., J. Biol. Chem., 244, 6049 (1969).

Application

Superoxide Dismutase (SOD) from bovine erythrocytes has been used:for measuring the superoxide radical using the electron paramagnetic resonance spin in human brain microvascular endothelial cellsfor measuring superoxide production in cytochrome C assay in peripheral blood mononuclear cellsas a standard in characterization of hen egg SOD using Fourier-transform infrared spectroscopy (FTIR) and matrix-assisted laser desorption/ionization (MALDI) analysis

Biochem/physiol Actions

Catalyzes the dismutation of superoxide radicals to hydrogen peroxide and molecular oxygen. Plays a critical role in the defense of cells against the toxic effects of oxygen radicals. Competes with nitric oxide (NO) for superoxide anion (which reacts with NO to form peroxynitrite), thereby SOD promotes the activity of NO. SOD has also been shown to suppress apoptosis in cultured rat ovarian follicles, neural cell lines, and transgenic mice.

General description

Superoxide Dismutase from bovine erythrocytes is a metalloprotein which disproportionates superoxide anion radicals. It is a 31.5 kDa copper binding protein and displays a conserved domain and fold. It is a homodimer with one copper and zinc ion per subunit and has antiparallel “greek-key” ? barrel fold.

Packaging

15000 units in glass bottle

30000, 75000 units in poly bottle

Unit Definition

One unit will inhibit reduction of cytochrome c by 50% in a coupled system with xanthine oxidase at pH 7.8 at 25 °C in a 3.0 mL reaction volume. Xanthine oxidase concentration should produce an initial δA₅₅₀of 0.025 ± 0.005 per min.

Specifications

No detailed specifications are available for this product.

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Code	Description	Quality Level	pH	Manufacturer	Product Line	Form	Price	Quantity
3572259	Superoxide Dismutase from bovine erythrocytes, BioReagent, >=3,000 units/mg protein, suitable for ce	200	7.6-10.5	SIGMA-ALDRICH	BioReagent	lyophilized powder	£94.25 (exc VAT) per EACH	- +
3572260	Superoxide Dismutase from bovine erythrocytes, BioReagent, >=3,000 units/mg protein, suitable for ce	200	7.6-10.5	SIGMA-ALDRICH	BioReagent	lyophilized powder	£163.75 (exc VAT) per EACH	- +
3572261	Superoxide Dismutase from bovine erythrocytes, BioReagent, >=3,000 units/mg protein, suitable for ce	200	7.6-10.5	SIGMA-ALDRICH	BioReagent	lyophilized powder	£328.75 (exc VAT) per EACH	- +