This product is tested for its ability to support the development of one-cell mouse embryos to expanded blastocysts. Minimum requirement is 80% development to blastocyst.
Application
M2 and M16 Medium are common media for in vitro culture of preimplantation stage embryos. It is a modified Krebs-Ringer bicarbonate solution, which is very similar to Whitten′s Medium. M16 contains pyruvate and lactate as energy sources since preimplantation embryos do not utilize glucose efficiently.
Quality
Presence of particulates in solution will not affect product′s performance.
Reconstitution
Supplement with 0.06 g/L potassium penicillin-G and 0.05 g/L streptomycin sulfate.