L-Glutamine, gamma-irradiated, BioXtra, suitable for cell culture
Application
L-Glutamine has been used as a media supplement:for cochlear organotypic culturingin Iscove?s modified Dulbecco?s medium for HeLa cells culturingin Dulbecco?s modified Eagle?s medium for primary hepatocyte cell culturing
Biochem/physiol Actions
Glutamine mediates protein turnover via cellular mTOR (mammalian target of rapamycin) signalling. It is also known to be associated with the inhibition of apoptosis.
L-glutamine is an essential amino acid that is a crucial component of culture media, and serves as a major energy source for cells in culture. Optimal cell performance usually requires supplementation of the medium with L-glutamine prior to use.
L-Glutamine is an essential amino acid that is a crucial component of culture media that serves as a major energy source for cells in culture. L-Glutamine is very stable as a dry powder and as a frozen solution. In liquid media or stock solutions, however, L-glutamine degrades relatively rapidly. Optimal cell performance usually requires supplementation of the media with L-glutamine prior to use.
Other Notes
The dipeptide L-Ala-LGln is frequently used in modern serum-free media formulations because it provides a more stable form of glutamine in vitro.
Packaging
50 mL amber serum vial with rubber stopper.
Reconstitution
Prepares a 200 mM solution when reconstituted to 10 mL with sterile water.
Suitability
Qualified for use in cell culture applications, especially serum-free cell culture.
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