Thymidine, powder, BioReagent, suitable for cell culture
Application
Thymidine can be used in HT and HAT cocktails for hybridoma fusion, selection and cloning. It has been used:as a nucleotide source in minimal essential medium to evaluate the outgrowth of the iSCNT-derived blastocystsas a supplement in Dulbecco?s Modified Eagle Medium (DMEM)selection medium used for the production of anti-neurogranin (NRGN) antibodiesto prepare THMG (thymidine, hypoxanthine, glycine, methotrexate) and THG stock solution for cleansing mouse lymphoma cells
Biochem/physiol Actions
Thymidine is useful in cell synchronization during S-phase. In the salvage pathway of pyrimidines, pyrimidine phosphorylase reversibly converts thymine to thymidine.
General description
Thymidine is also referred to as pyrimidine deoxynucleoside. Deoxythymidine is a nucleoside present in DNA. In a DNA double stranded structure, thymidine pairs with deoxyadeninosine.
Packaging
1, 5, 10, 25 g in poly bottle
| Pack | 25G |
|---|---|
| mp | 186-188 °C (lit.) |
| InChI key | IQFYYKKMVGJFEH-XLPZGREQSA-N |
| InChI | 1S/C10H14N2O5/c1-5-3-12(10(16)11-9(5)15)8-2-6(14)7(4-13)17-8/h3,6-8,13-14H,2,4H2,1H3,(H,11,15,16)/t6-,7+,8+/m0/s1 |
| Manufacturer | SIGMA-ALDRICH |
| Product Line | BioReagent |
| Form | powder |
| Assay | ≥99% |
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