Streptomycin sulfate salt, powder, BioXtra, suitable for mouse embryo cell culture

Streptomycin sulfate salt, powder, BioXtra, suitable for mouse embryo cell culture

Stock Code: 3572238
Manufacturer Part No: S1277-50G
Order Now for 21 day delivery
£269.80 (exc VAT) per 50G
Quantity: - +

Application


Streptomycin is used to study mechanisms of streptomycin resistance and is often used together with penicillin and other agents to inhibit bacterial contamination in cell culture applications. It is recommended for use in molecular biology applications at 25-50µg/mL, in cell culture applications at 100 mg/L and in embryo culture at 50 mg/L.


Biochem/physiol Actions


Mode of Action: Streptomycin acts by inhibiting prokaryote protein synthesis by binding to the S12 protein of the 30S ribosomal subunit, thus preventing the transition from imitation complex to chain-elongating ribosome. This causes miscoding or inhibits initiation.Mode of Resistance: A mutation in rpsL, a gene for S12 ribosomal protein, prevents binding of streptomycin to the ribosome. An aminoglycoside phosphotransferase also inactivates streptomycin.Antimicrobial spectrum: Streptomycin acts against gram-negative and gram-positive bacteria.


Caution


Solutions can be stored at 2-8°C for up to a month or at -20°C for more extended periods. At 37°C, solutions are stable for three days.


General description


Chemical structure: aminoglycoside


Packaging


5, 50 g in poly bottle


Preparation Note


Stock solutions should be prepared with sterile water at concentrations between 20-25 mg/ml.

Quality Level200
Pack50G
InChI keyQTENRWWVYAAPBI-YZTFXSNBSA-N
InChI1S/2C21H39N7O12.3H2O4S/c2*1-5-21(36,4-30)16(40-17-9(26-2)13(34)10(31)6(3-29)38-17)18(37-5)39-15-8(28-20(24)25)11(32)7(27-19(22)23)12(33)14(15)35;3*1-5(2,3)4/h2
ManufacturerSIGMA-ALDRICH
Product LineBioXtra
Formpowder

There are no downloads for this product.