Sodium bicarbonate solution has been used:as a component of Hanks? buffered salt solution (HBSS) and recording medium for culturing of tissue explants from rats or miceas a component of Dulbecco?s modified Eagle?smedium (DMEM) to culture mice tissue explantsas a component of Minimum Essential Media (MEM) to study the cytotoxicity against human colorectal adenocarcinoma cells and mammary gland adenocarcinoma