SILU(TM)MAB K1 STABLE-ISOTOPE LABELED

SILU(TM)MAB K1 STABLE-ISOTOPE LABELED

Stock Code: 3587195
Manufacturer Part No: MSQC6-5MG
Order Now for 7 day delivery
Click Add to Quote for price and delivery
Quantity: - +

Analysis Note


SILuMAb K1 Heavy ChainEVQLVESGGGLVQPGGSLRLSCVASGFTLNNYDMHWVRQGIGKGLEWVSKIGTAGDRYYAGSVKGRFTISRENAKDSLYLQMNSLRVGDAAVYYCARGAGRWAPLGAFDIWGQGTMVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPG SILuMAb K1 Light ChainQSALTQPRSVSGSPGQSVTISCTGTSSDIGGYNFVSWYQQHPGKAPKLMIYDATKRPSGVPDRFSGSKSGNTASLTISGLQAEDEADYYCCSYAGDYTPGVVFGGGTKLTVLTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGECTarget overlap areas are underlinedPackage size based on protein content determined by A280 using an extinction coefficient (E0.1%) of 1.4


QuantitativeMRM settings provided (xls)


Application


SILu™ MAB K1 Stable-Isotope Labeled Universal Monoclonal Antibody has been used to spike peptide samples to assess preparation reproducibility.


Features and Benefits


Universal Peptide Sequence LocationFNWYVDGVEVHNAKHeavy Chain (IgG1)VVSVLTVLHQDWLNGKHeavy Chain (IgG1, IgG3, IgG4)GFYPSDIAVEWESNGQPENNYKHeavy Chain (IgG1, IgG4)SGTASVVCLLNNFYPRLight Chain (kappa)VDNALQSGNSQESVTEQDSKLight Chain (kappa)DSTYSLSSTLTLSKLight Chain (kappa)SILu™Mab has been validated as an internal standard for quantitation of relevant biotherapeutics in a complex biological matrix by MRM-based LC-MS/MS. •SILu™Mab yielded reproducible, linear curves from 0.1 μg/mL to 1000 μg/mL without enrichment or depletion. •Good agreement was observed between multiple peptides derived from the same target. •Label incorporation was determined to be >98% by mass spectrometry. •Sequence coverage was confirmed by peptide mapping.


Legal Information


SILu is a trademark of Sigma-Aldrich Co. LLC


This product is licensed under U.S. Patent No. 7,396,688 and foreign counterparts from E. I. du Pont de Nemours and Company. The purchase of this product conveys to the buyer the nontransferable right to use the purchased amount of the product for research and development only, including services for a third party for consideration. The buyer cannot sell or otherwise transfer this product, its components or materials made using this product or its components to a third party. Information about licenses for excluded uses is available from: E. I. du Pont de Nemours and Company; Attn: Associate Director, Commercial Development; DuPont Experimental Station E268; 200 Powdermill Rd.; Wilmington, DE 19803; 1-877-881-9787 (voice), 1-302-695-1437 (fax), licensing@dupont.com.


Physical form


Supplied as a lyophilized powder containing phosphate buffered saline


Preparation Note


SILu™Mab design is optimized to be used as an internal standard for quantitation of monoclonal antibodies as well as Fc-fusion therapeutics

Quality Level200
ManufacturerSIGMA-ALDRICH
Storage Temp.−20°C
Assay≥90% (SDS-PAGE)

There are no downloads for this product.