Ribonuclease B from bovine pancreas, BioReagent, >=50 Kunitz units/mg protein, >=80% (SDS-PAGE)

Ribonuclease B from bovine pancreas, BioReagent, >=50 Kunitz units/mg protein, >=80% (SDS-PAGE)

Stock Code: 3587310
Manufacturer Part No: R7884-100MG
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Application


Ribonuclease B from bovine pancreas is used in the digestion of RNA during cell cycle platform analysis.


Biochem/physiol Actions


Native RNase BS generated by subtilisin digestion of native RNase B comprising of amino acid residues 21-124 of RNase B, is sensitive to PNGase F digestion. Intramolecular N-glycans of bovine pancreatic RNase B function like chaperone. RNase B is found to be much faster than RNase A, while RNase A is liable to aggregate during regeneration. The stimulatory effect of Asn-oligosaccharide (which corresponds to the most predominant sugar chain of RNase B) reveals that the N-glycans of RNase B facilitates the transformation of bulky intermediates into folded, compact species.


Packaging


100 mg in poly bottle


Package size based on protein content


500 µg in poly bottle


Preparation Note


Purified by affinity chromatography

Quality Level200
ManufacturerSIGMA-ALDRICH
Product LineBioReagent
Storage Temp.−20°C
Formpowder
Assay≥80% (SDS-PAGE)

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