Oleic acid, suitable for cell culture, BioReagent

Oleic acid, suitable for cell culture, BioReagent

Stock Code: 3572119
Manufacturer Part No: O1383-25G
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£791.25 (exc VAT) per 25G
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Application


Oleic acid has been used:in fatty acid oxidation rate experiments in myotubes and in Sirt1 enzyme assayto determine quantitatively its effect on lipid droplets by optical diffraction tomographyas a fatty acid source in the medium for primary hepatocyte culture


Biochem/physiol Actions


Activates protein kinase C in hepatocytes.


Uncouples oxidative phosphorylation. Inhibits 2,4-dinitrophenol−stimulated ATPase. Action reversed by adding serum albumin.


Caution


Unsaturated fatty acids are subject to oxidation and peroxidation. Consider complexed fatty acid supplements.


General description


Oleic acid mediates complete oxidation of fatty acid in sirtuin1- proliferator-activated receptor ? coactivator?1? (SIRT1-PGC-1?) signalling pathway. Oleic acid shows anti-inflammatory properties related to atherosclerosis and insulin resistant state. Oleic acid inhibits permeability in gap junctions and promotes glucose uptake in astrocytes. Oleic acid aids in neural cell proliferation by increasing glucose uptake and by protein kinase C (PKC) activation. Oleic acid is essential for axonal growth and neuronal clustering.


Oleic acid is a mono-unsaturated omega-9 fatty acid created by dehydrogenation of stearic acid. It is then further dehydrogenated and elongated to create members of the n-9 family of fatty acids. Oleic acid is not an essential fatty acid (EFA); however, providing it to cells in culture reduces energy demand.


Packaging


1, 5, 25 g in ampule


Sealed ampule.

Pack25G
InChI keyZQPPMHVWECSIRJ-KTKRTIGZSA-N
InChI1S/C18H34O2/c1-2-3-4-5-6-7-8-9-10-11-12-13-14-15-16-17-18(19)20/h9-10H,2-8,11-17H2,1H3,(H,19,20)/b10-9-
bp194-195 °C/1.2 mmHg (lit.)
ManufacturerSIGMA-ALDRICH
Density0.89 g/mL at 25 °C (lit.)
Formviscous liquid
Assay≥99% (GC)

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