Monoclonal ANTI-FLAG M2-Cy3 antibody produced in mouse, clone M2, purified immunoglobulin, buffered

Monoclonal ANTI-FLAG M2-Cy3 antibody produced in mouse, clone M2, purified immunoglobulin, buffered

Stock Code: 3586589
Manufacturer Part No: A9594-5X1MG
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Application


For simple, one-step detection by immunocytochemistry. Especially useful in detection of FLAG fusion proteins expressed in murine host, where secondary anti-mouse antibodies might cause cross-reactivity.Browse additional application references in our FLAG® Literature portal.


Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.Western Blotting (1 paper)


General description


Monoclonal ANTI-FLAG M2-Cy3 (mouse IgG) antibody is covalently conjugated to cyanine dye Cy3. The antibody conjugate binds to FLAG fusion proteins, and will recognize the FLAG sequence at the N-terminus, Met-N-terminus, or C-terminus of FLAG fusion proteins.


Legal Information


Cy3 is a trademark of Cytiva


FLAG is a registered trademark of Sigma-Aldrich Co. LLC


ANTI-FLAG is a registered trademark of Sigma-Aldrich Co. LLC


Other Notes


Suggested concentration of 1-10 mg/ml for immunocytochemistry.


Packaging


0.2, 1 mg in poly bottle


Physical form


Solution in phosphate buffered saline plus 1% BSA and preservative


Preparation Note


Dilute the antibody in Tris buffered saline (TBS): 0.05 M Tris,pH 7.4, with 0.15 M NaCl.

ManufacturerSIGMA-ALDRICH
Formbuffered aqueous solution (Supplied as a solution in 10 mM sodium phosphate)

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