M2 medium, With HEPES, without penicillin and streptomycin, liquid, sterile-filtered, suitable for m

M2 medium, With HEPES, without penicillin and streptomycin, liquid, sterile-filtered, suitable for m

Stock Code: 3571977
Manufacturer Part No: M7167-50ML
Order Now for 21 day delivery
£49.08 (exc VAT) per 50ML
Quantity: - +

Analysis Note


This product is tested for its ability to support the development of one-cell mouse embryos to expanded blastocysts. Minimum requirement is 80% development to blastocyst.


Application


Recommended for manipulation of mouse embryos at ambient temperature. M2 and M16 Medium are common media for in vitro culture of preimplantation stage embryos. It is a modified Krebs-Ringer bicarbonate solution, which is very similar to Whitten′s Medium. M16 contains pyruvate and lactate as energy sources since preimplantation embryos do not utilize glucose efficiently.M2 Medium is a further modification of M16 that substitutes HEPES buffer in place of some of the bicarbonate. M2 is used for collecting and handling embryos for prolonged periods outside a CO2 incubator.


Quality


Presence of particulates in solution will not affect product′s performance.


Reconstitution


Supplement with 0.06 g/L potassium penicillin-G and 0.05 g/L streptomycin sulfate.

Quality Level400
Pack50ML
ManufacturerSIGMA-ALDRICH
Storage Temp.2-8°C
Impuritiesendotoxin, tested
Formliquid

There are no downloads for this product.