Lectin from Triticum vulgaris (wheat), peroxidase conjugate, lyophilized powder

Lectin from Triticum vulgaris (wheat), peroxidase conjugate, lyophilized powder

Stock Code: 3587071
Manufacturer Part No: L3892-2MG
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£561.32 (exc VAT) per 2MG
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Application


Detects glycoproteins containing β(1→4)-N-acetyl-D-glucosamine when used with appropriate peroxidase substrate


General Western Blot Protocol: Glycoprotein sample size: 500ng Lectin Concentration: 0.1ug/mlLoad samples at 500 ng of glycoprotein per lane Run 4-20% Bis-Tris SDS page gel Transfer gel to a PVDF membrane Block membrane for 1 hr at RT with RIPA buffer (R0278 Sigma) Incubate HRP lectin at 0.1ug/ml with RIPA buffer for 2 hours at RT Wash membrane 5 x 5 minutes with 25ml RIPA buffer Detect using chemiluminescent substrate (CPS1-120)


Biochem/physiol Actions


WGA is not blood group specific but has an affinity for N-acetyl-β-D-glucosaminyl residues and N-acetyl-β-D-glucosamine oligomers. WGA contains no protein-bound carbohydrate.


Packaging


1 mg in glass bottle


Package size based on protein content.


2 mg in serum bottle


Physical form


Contains citrate buffer


Preparation Note


Prepared from peroxidase type VI (P8375) using a modification of the method of O?Sullivan, which favors low molecular weight conjugates. Repurified after conjugation by affinity chromatography.


Conjugates are prepared from affinity purified lectin.


Unit Definition


One unit will form 1.0?mg of purpurogallin from pyrogallol in 20 sec at pH?6.0 at 20?°C.

Quality Level200
ManufacturerSIGMA-ALDRICH
CompositionProtein, ~90% modified Warburg-Christian
Formlyophilized powder
Assay≥85% protein basis (Warburg-Christian)

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