Application
Lectin from Triticum vulgaris (wheat) has been used: to treat transfected cells for immunofluorescence to stereotaxically inject into mice for neuronal tracing in immunohistochemistry for the analysis of myocytes
For the fluorescent detection of glycoproteins containing β(1→4)-N-acetyl-D-glucosamine
Biochem/physiol Actions
Lectin plays a key role in glycoconjugate characterizing, imaging and targeting. Its use in a microarray assay, enable efficient glycome profiling. In plants and fungi, lectin defends against pathogens/feeders. Lectin also plays a major role in host recognition and tissue adhesion.
WGA is not blood group specific but has an affinity for N-acetyl-β-D-glucosaminyl residues and N-acetyl-β-D-glucosamine oligomers. WGA contains no protein-bound carbohydrate.
General description
Lectins are carbohydrate-binding proteins. They are omnipresent and usually found in fungi, plants and animals. The secondary structure of this protein is rich in ?-strands and possesses carbohydrate binding sites on the surface.
Packaging
Package size based on protein content.
2, 5, 10 mg in glass bottle
Physical form
Contains phosphate buffer salts
Preparation Note
Conjugates are prepared from affinity purified lectin.