Lectin from Pseudomonas aeruginosa, lyophilized powder

Stock Code: 3587131
Manufacturer Part No: L9895-2MG

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Descriptions

Analysis Note

Agglutination activity is expressed in µg/ml and is determined from serial dilutions of a 1 mg/ml solution using phosphate buffered saline, pH 7.0. This activity is the lowest concentration to agglutinate a 2% suspension of neuraminidase treated human blood group A erythrocytes after one hr incubation at 25 °C.

Biochem/physiol Actions

PA-I has a major affinity for D-galactose and its derivatives. PA-I agglutinates all types of human and animal erythrocytes and interacts also with unicellular protozoa, algae and bacteria. PA-I induces mitogenic stimulation in cultured human lymphocytes pretreated with neuraminidase.

Packaging

Package size based on protein content. Vial content based on protein by biuret.

1, 2 mg in glass bottle

Physical form

Lyophilized powder containing approx. 80% protein (biuret), balance primarily sodium citrate, plus traces of calcium chloride, magnesium chloride and manganese chloride

Preparation Note

Purified by affinity chromatography using a modification of Gilboa-Garber.¹

Quality

Highly purified.