L-Glutamine, gamma-irradiated, BioXtra, suitable for cell culture

L-Glutamine, gamma-irradiated, BioXtra, suitable for cell culture

Stock Code: 3571733
Manufacturer Part No: G6392-1VL
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Application


L-Glutamine has been used as a media supplement:for cochlear organotypic culturingin Iscove?s modified Dulbecco?s medium for HeLa cells culturingin Dulbecco?s modified Eagle?s medium for primary hepatocyte cell culturing


Biochem/physiol Actions


Glutamine mediates protein turnover via cellular mTOR (mammalian target of rapamycin) signalling. It is also known to be associated with the inhibition of apoptosis.


L-glutamine is an essential amino acid that is a crucial component of culture media, and serves as a major energy source for cells in culture. Optimal cell performance usually requires supplementation of the medium with L-glutamine prior to use.


L-Glutamine is an essential amino acid that is a crucial component of culture media that serves as a major energy source for cells in culture. L-Glutamine is very stable as a dry powder and as a frozen solution. In liquid media or stock solutions, however, L-glutamine degrades relatively rapidly. Optimal cell performance usually requires supplementation of the media with L-glutamine prior to use.


Other Notes


The dipeptide L-Ala-LGln is frequently used in modern serum-free media formulations because it provides a more stable form of glutamine in vitro.


Packaging


50 mL amber serum vial with rubber stopper.


Reconstitution


Prepares a 200 mM solution when reconstituted to 10 mL with sterile water.


Suitability


Qualified for use in cell culture applications, especially serum-free cell culture.

Colourwhite to faint yellow
mp185 °C (dec.) (lit.)
InChI keyZDXPYRJPNDTMRX-VKHMYHEASA-N
InChI1S/C5H10N2O3/c6-3(5(9)10)1-2-4(7)8/h3H,1-2,6H2,(H2,7,8)(H,9,10)/t3-/m0/s1
ManufacturerSIGMA-ALDRICH
Impuritiesendotoxin, tested
Formpowder

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