holo-Transferrin human, powder, BioReagent, suitable for cell culture, >=97%

holo-Transferrin human, powder, BioReagent, suitable for cell culture, >=97%

Stock Code: 3572642
Manufacturer Part No: T0665-500MG
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£773.57 (exc VAT) per 500MG
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Analysis Note


Iron content by ICP reported on a lot-specific basis.


Purity by agarose gel electrophoresis.


Application


Transferrin is the primary iron binding and cell delivery molecule present in serum. Serum-free cell culture systems require a delivery format for iron. Transferrin is the preferential delivery form of iron because cells process transferrin bound iron in a physiologically appropriate way through transferrin receptors on the cell surface. Human holo-transferrin is a high affinity transferrin that can be used with a wide range of cells types. Human holo-transferrin is loaded with iron prior to use and can be added directly to iron poor or iron-free cell culture media.


holo-Transferrin human has been supplemented in Roswell Park Memorial Institute (RPMI) for proerythroblasts proliferation assay and also in culture media for inductively coupled plasma emission spectroscopy.


Biochem/physiol Actions


Provides iron to cells in a physiologically stable and safe form.


Packaging


1 g in poly bottle


50, 100, 500 mg in poly bottle


Preparation Note


Heat treated at 60 °C (minimum) for a minimum of 10 hours.

Quality Level200
Pack500MG
ManufacturerSIGMA-ALDRICH
Further InfoIron-saturated
Product LineBioReagent
Impuritiesendotoxin, tested, HIV and HBsAg, none detected
Formpowder
Assay≥97%

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