Histopaque-1077 Hybri-Max, liquid, sterile-filtered, BioReagent, suitable for hybridoma
Application
Histopaque®-1077 Hybri-Max™ has been used to isolate: peripheral blood mononuclear cells (PBMCs) from heparinized blood by gradient centrifugationimmune cells (lymphocytes) from peripheral bloodPBMCs from blood
General description
Histopaque®-1077 is a solution comprising polysucrose and sodium diatrizoate, adjusted to a density of 1.077 +/- 0.001 g/cm3. This medium facilitates rapid recovery of viable lymphocytes from small volumes of whole blood. This technique is suitable for use in cell-mediated lympholysis and human lymphocyte antigen (HLA)2 typing. It may also be employed as the initial isolation step before the enumeration of T-, B-, and null lymphocytes. It is used to create a density medium for the purification of lymphocytes and other mononuclear cells.
Legal Information
Hybri-Max is a trademark of Sigma-Aldrich Co. LLC
Histopaque is a registered trademark of Sigma-Aldrich Co. LLC
| Quality Level | 400 |
|---|---|
| Pack | 100ML |
| Manufacturer | SIGMA-ALDRICH |
| Technique(s) | cell culture - hybridoma: suitable |
| Product Line | BioReagent |
| Storage Temp. | 2-8°C |
| Impurities | endotoxin, tested |
| Density | 1.077 g/cm3 at 25 °C (lit.) |
| Form | liquid |
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