HEPES solution, 1 M, pH 7.0-7.6, sterile-filtered, BioReagent, suitable for cell culture

HEPES solution, 1 M, pH 7.0-7.6, sterile-filtered, BioReagent, suitable for cell culture

Stock Code: 3571847
Manufacturer Part No: H0887-20ML
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Application


HEPES has been used as a component of:Hank’;s balanced salt solution, Dulbecco’;s modified eagle’;s medium and no-glucose DMEM, which are used for the preparation of tissue slicesBuffers used in localized conductance measurements on the basolateral side of culture inserts to examine cation selectivity


Caution


Concentrations of HEPES over 40mM may be toxic to some cell types.


General description


HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2 (pKa 7.55). It is typically used in cell culture at concentration between 5mM to 30 mM. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.


Preparation Note


Prepared from HEPES free acid and cell culture grade water.

Quality Level300
Pack20ML
pH7.0-7.6
InChI keyJKMHFZQWWAIEOD-UHFFFAOYSA-N
InChI1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
ManufacturerSIGMA-ALDRICH
Product LineBioReagent
Storage Temp.2-8°C
Impuritiesendotoxin, tested
Formliquid

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