GenElute#-E RNA Cleanup Kit

GenElute#-E RNA Cleanup Kit

Stock Code: 3586875
Manufacturer Part No: EC800-50RXN
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£245.14 (exc VAT) per EACH
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Application


Traditional methods for purifying genomic RNA rely on organic solvents or medium and large format silica (bind-wash-elute) purification kits, which suffer from limited binding capacity, low RNA concentration, and low purity. The GenElute™-E Single Spin RNA Clean-up Kit, depletes impurities such as salts, detergents, nucleotides, primers, proteins/enzymes, organic solvents, and dyes from RNA solutions. Purified RNA is eluted in Tris buffer, pH 7.8 and can immediately be used for most common downstream applications, including RT-PCR, genotyping, NGS, and others.


Use within 12 months of receipt.


Features and Benefits


Fast and efficient purification (less than 3 minutes): single-spin protocol reduces pipetting steps and hands-on timeHigher quality RNA: negative chromotography-based purification reduces carryover of RT-PCR inhibitors and other contaminents Reduced environmental impact: 55% less plastic waste, as well as a significant reduction in hazardous chemicals


General description


We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is aligned with "Waste prevention" and "Designing safer chemicals". Click here for more information.


DNA/RNA preparation by traditional methods frequently contains residual phenol and/or chloroform. The GenElute™-E Single Spin RNA Cleanup Kit is a one-step cleanup kit for the depletion of organic solvent traces, including phenol, TRIzol reagent, chloroform, and ethanol, from RNA solutions. Ideally suited for a reliable clean-up when a universal preparation for NGS library prep is desired or required, or for RNA cleanup following silica-based isolation to remove process contaminantsGenElute™-E kits are based on the principle of negative chromatography, where impurities like salt, detergent, proteins and organic solvents are removed by the column material and the purified nucleic acid is collected in the flow-through in just one single centrifugation step. This convenient procedure enables a significantly faster purification of nucleic acid samples than standard bind-wash-elute procedures, with less handling, drastically reduced plastic consumable usage, and no use of hazardous materials like chaotropic salts or organic solvents.With conventional methods, traces of chaotropic salt and ethanol are usually coeluted with these purification procedures, which often leads to inhibition of real-time PCR experiments. GenElute™-E kits do not contain any chaotropic salts, organic solvents or EDTA, resulting in improved performance in downstream applications such as RT-PCR, gene expression, and NGS.Because of the negative chromatography principle, there is no limitation to the amount of DNA that is cleaned up. Maximum sample volume is 100 µl. The purified nucleic acid is eluted in water and can immediately be used for downstream applications. A 1x TE Buffer, pH 8.5, is supplied to enable storage of samples.


Legal Information


GenElute is a trademark of Sigma-Aldrich Co. LLC


Other Notes


We believe that green chemistry will contribute to a better tomorrow

ManufacturerSIGMA-ALDRICH
FeatureCompatible Application (Suitable for most common downstream applications, including RT-PCR, gene expression, and NGS), Typical/expected yield (Varies by sample
Storage Temp.room temp

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