Elastase from porcine pancreas, lyophilized powder, BioReagent, suitable for cell culture

Elastase from porcine pancreas, lyophilized powder, BioReagent, suitable for cell culture

Stock Code: 3571592
Manufacturer Part No: E7885-20MG
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£547.50 (exc VAT) per 20MG
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Application


Elastase from porcine pancreas is used for the following applications:Cell culture techniquesELISA techniquesUsed in assay for elastase inhibitory activity


Biochem/physiol Actions


Enzyme that degrades elastin, a matrix component of tissues involved in stretching processes. It is a serine protease with broad specificity. It cleaves protein at the carboxyl side of small hydrophobic amino acids such as Ile, Gly, Ala, Ser, Val, and Leu. The enzyme also hydrolyzes amides and esters such as N-Benzoyl-L-alanine methyl ester.


Elastase hydrolyses elastin, the specific protein of elastic fibers, and digests hemoglobin, casein and fibrin.


General description


Elastase is a single polypeptide chain of 240 amino acid residues and contains four disulfide bridges. The molecular mass is approximately 25.9kDa. The enzyme is synthesized as an inactive zymogen, proelastase, which is converted to the active form by limited proteolysis at the N-terminal by trypsin.


Packaging


Package size based on protein content


1, 5 mg in glass bottle


20 mg in poly bottle


Unit Definition


One unit will hydrolyze 1.0 μmole of N-succinyl-L-Ala-Ala-Ala-p-nitroanilide per min, pH 8.0 at 25°C.

Quality Level200
Pack20MG
pH8
ManufacturerSIGMA-ALDRICH
Formlyophilized powder

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