Concanavalin a type iv

Stock Code: 3586669
Manufacturer Part No: C2010-250MG
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Analysis Note


Where reported, agglutination activity is expressed in µg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, containing Ca2+ and Mn2+ of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C.


Application


Concanavalin A from Canavalia ensiformis (Jack bean) has been used:as T-lymphocyte mitogen in lymphocyte proliferation assay for correlations with organochlorine contaminants (OCs)for in vitro stimulation of T cell hybridomas, BW5147 cells and primary CD4+ T cells for T cell vaccination and for induction of hepatitisfor the stimulation of mice splenocytes in the suppression of T-lymphocytes proliferation assayfor the stimulation of spleen in mitogen assays


Biochem/physiol Actions


Con A is not blood group specific but has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. Ca2+ and Mn2+ ions are required for activity. Con A dissociates into dimers at pH 5.6 or below. Between pH 5.8 and pH 7.0, Con A exists as a tetramer; above pH 7.0 higher aggregates are formed. Con A exhibits mitogenic activity which is dependent on its degree of aggregation. Succinylation results in an active dimeric form which remains a dimer above pH 5.6.


General description


Concanavalin A(con A) is a plant lectin refined from jack beans. It is a tetrameric protein, which has four identical 28 kDa subunits. Con A has the ability to connect with the mannose residues of several glycoproteins and can stimulate lymphocytes.


Packaging


1 g in glass bottle


100, 250 mg in glass bottle


25 mg in poly bottle


Quality


Highly purified by specific affinity chromatography

Quality Level300
ManufacturerSIGMA-ALDRICH
Storage Temp.−20°C
Impuritiessalt, essentially free, Carbohydrate, essentially free
Formlyophilized powder

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