Concanavalin A from Canavalia ensiformis (Jack bean), FITC conjugate, Type IV, lyophilized powder

Stock Code: 3586702
Manufacturer Part No: C7642-10MG

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Descriptions

Analysis Note

Where reported, agglutination activity is expressed in µg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, containing Ca²⁺ and Mn²⁺ of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C.

Application

For the fluorescent detection of glycoproteins containing α−D-mannose or α−D-glucose

Biochem/physiol Actions

Con A is not blood group specific but has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. Ca²⁺ and Mn²⁺ ions are required for activity. Con A dissociates into dimers at pH 5.6 or below. Between pH 5.8 and pH 7.0, Con A exists as a tetramer; above pH 7.0 higher aggregates are formed. Con A exhibits mitogenic activity which is dependent on its degree of aggregation. Succinylation results in an active dimeric form which remains a dimer above pH 5.6.

Packaging

2, 10 mg in glass bottle

Package size based on protein content

Physical form

Lyophilized powder containing phosphate buffer salts and NaCl

Specifications

Quality Level	200
Manufacturer	SIGMA-ALDRICH
Composition	Protein, 5-15% biuret
Storage Temp.	−20°C
Impurities	Carbohydrate, essentially free
Form	lyophilized powder