apo-Transferrin human, powder, BioReagent, suitable for cell culture, >=98% (agarose gel electrophor
Application
Apo-Transferrin human can be supplemented with iron or used to bind free iron present in media. It is also used to transport iron into mammalian cells.
Biochem/physiol Actions
Transferrin is the primary iron binding and cell delivery molecule present in serum. Serum-free cell culture systems require a delivery format for iron. Transferrin is the preferential delivery form of iron because cells process transferrin bound iron in a physiologically appropriate way through transferrin receptors on the cell surface. Human apo-transferrin is a high affinity transferrin that can be used with a wide range of cells types. Apo-transferrin can be loaded with iron prior to use or added directly to an iron containing medium.
Other Notes
Non-heme iron-transport protein.
Packaging
100, 5×100, 500 mg in poly bottle
1, 5 g in poly bottle
| Quality Level | 200 |
|---|---|
| Pack | 100MG |
| Manufacturer | SIGMA-ALDRICH |
| Product Line | BioReagent |
| Storage Temp. | −20°C |
| Impurities | HIV and HBsAg, source material tested negative; ≤0.005% iron |
| Solubility | H2O: 50 mg/mL |
| Form | powder |
| Assay | ≥98% (agarose gel electrophoresis) |
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