4,6-Diamidino-2-phenylindole dihydrochloride, powder, BioReagent, suitable for cell culture, >=98% (

4,6-Diamidino-2-phenylindole dihydrochloride, powder, BioReagent, suitable for cell culture, >=98% (

Stock Code: 3571572
Manufacturer Part No: D8417-1MG
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£54.01 (exc VAT) per 1MG
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Application


DAPI is several times more sensitive than ethidium bromide for staining DNA in agarose gels. It may be used for photofootprinting of DNA, to detect annealed probes in blotting applications by specifically visualizing the double-stranded complex, and to study the changes in DNA and analyze DNA content during apoptosis using flow cytometry. DAPI staining has also been shown to be a sensitive and specific detection method for mycoplasma.


4?,6-Diamidino-2-phenylindole dihydrochloride has been used in staining of various cells in immunocytochemistry and cytochemistry, human subcutaneous adipose tissues for fluorescence imaging, embryo sections for immunofluorescence.


Biochem/physiol Actions


Cell permeable fluorescent minor groove-binding probe for DNA. Binds to the minor groove of double-stranded DNA (preferentially to AT rich DNA), forming a stable complex which fluoresces approximately 20 times greater than DAPI alone.


Caution


Protect from light.


General description


4?,6-Diamidino-2-phenylindole dihydrochloride is a fluorescent dye for staining DNA. It strongly interacts with the dAT-rich (deoxy adenine-thymine) regions of the DNA.


Packaging


1, 5, 10 mg in glass bottle

Quality Level200
Pack1MG
InChI keyFPNZBYLXNYPRLR-UHFFFAOYSA-N
InChI1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H
ManufacturerSIGMA-ALDRICH
Product LineBioReagent
Formpowder
Assay≥98% (HPLC and TLC)

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