Lectin from Phaseolus vulgaris (red kidney bean), Phytohemagglutinin PHA-M, lyophilized powder, BioR

Lectin from Phaseolus vulgaris (red kidney bean), Phytohemagglutinin PHA-M, lyophilized powder, BioR

Stock Code: 3587114
Manufacturer Part No: L8902-25MG
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Analysis Note


Agglutination activity is expressed in µg/mL and is determined from serial dilutions of a 1 mg/mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes in phosphate buffered saline, pH 6.8, after 1 hr incubation at 25 °C.


Application


Lectin from Phaseolus vulgaris (red kidney bean) is used in Lymphocyte Transformation Assay and culture medium.


Biochem/physiol Actions


PHA consists of two molecular species, an erythroagglutinin (PHA-E) which has low mitogenic activity and high erythroagglutinin activity, and leucoagglutinin (PHA-L) which has high mitogenic and leucoagglutinating activity, but very low erythroagglutinating activity. PHA-E is not blood group specific, but agglutination can be inhibited by certain oligosaccharides. PHA-P is the protein form of PHA prior to separation and purification of erythroagglutinin and leucoagglutinin. PHA-M is the mucoprotein form. Conjugates are prepared from the corresponding purified lectins.


Packaging


25, 100 mg in glass bottle

Quality Level200
ManufacturerSIGMA-ALDRICH
Technique(s)cell culture - mammalian: suitable
Product LineBioReagent
Storage Temp.2-8°C
Impuritiessalt, free
SolubilityPBS: 2 mg/mL
Formlyophilized powder

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