Chromogenic substrate for β-glucuronidase (GUS) gene detection.
5-Bromo-4-chloro-3-indolyl ?-D-glucuronide sodium salt has been used:as a component in ?-glucuronidase (GUS) assay buffer for histochemical GUS assayto prepare X-Gluc or GUS staining solutionto confirm GUS expression in samples by X-gluc staining
Biochem/physiol Actions
5-bromo-4-chloro-3-indolyl-?-D-glucuronide sodium salt (X-GlcA), a colorless substrate for ?-glucuronidase enzyme, generates a blue color at the end of the reaction.