o-Dianisidine dihydrochloride, tablet, 10 mg substrate per tablet
Packaging
50, 100 tablets in glass bottle
Reconstitution
Dissolve one tablet in 60 ml of 50 mM phosphate-citrate buffer, pH 5.0. Add 12 µl of fresh 30% hydrogen peroxide immediately before to use.
Specificity
o-Dianisidine (3,3′-dimethoxybenzidine) is a peroxidase substrate suitable for use in ELISA procedures. This substrate produces a soluble end product that is yellow-orange in color and can be read spectrophotometrically at 405 nm. The reaction may be stopped with 5 M HCl.
Warning
Probably carcinogenic.
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