4-Nitrophenyl phosphate disodium salt hexahydrate, tablet

Application

4-Nitrophenyl phosphate disodium salt hexahydrate has been used in enzyme-linked immunosorbent assay (ELISA) as a substrate for streptavidin-Alkaline Phosphatase. It has also been used as a substrate in control assay for comparing 4-aminophenyl phosphate/diethanolamine (APP/DEA) assay for alkaline phosphatase.

Biochem/physiol Actions

4-Nitrophenyl phosphate disodium salt hexahydrate (pNPP) is a non-proteinaceous and non-specific substrate for determining protein tyrosine phosphatase activity. Hydrolysis of pNPP yields p-nitrophenol. pNPP is a common chromogenic substrate for alkaline phosphatase.

General description

Chromogenic substrate for the determination of acid and alkaline phosphatases. The reaction produces a soluble end product that is yellow in color. The reaction may be stopped with 3 N NaOH and read at 405 nm.

Quantity

Contains 15 mg substrate per tablet

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Code Description Quality Level InChI key InChI Manufacturer Further Info Storage Temp. Solubility Form Price
3578536 4-Nitrophenyl phosphate disodium salt hexahydrate, tablet 200 KAKKHKRHCKCAGH-UHFFFAOYSA-L 1S/C6H6NO6P.2Na.6H2O/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;;;;;;;/h1-4H,(H2,10,11,12);;;6*1H2/q;2*+1;;;;;;/p-2 SIGMA-ALDRICH - −20°C water: 1 tablet/3 mL, clear, colorless tablet
3578537 4-Nitrophenyl phosphate disodium salt hexahydrate, tablet 200 KAKKHKRHCKCAGH-UHFFFAOYSA-L 1S/C6H6NO6P.2Na.6H2O/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;;;;;;;/h1-4H,(H2,10,11,12);;;6*1H2/q;2*+1;;;;;;/p-2 SIGMA-ALDRICH - −20°C water: 1 tablet/3 mL, clear, colorless tablet
3578540 4-Nitrophenyl phosphate disodium salt hexahydrate, tablet 300 KAKKHKRHCKCAGH-UHFFFAOYSA-L 1S/C6H6NO6P.2Na.6H2O/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;;;;;;;/h1-4H,(H2,10,11,12);;;6*1H2/q;2*+1;;;;;;/p-2 SIGMA-ALDRICH - −20°C water, high purity: 0.25 tablet/mL, clear, colorless tablet
3578541 4-Nitrophenyl phosphate disodium salt hexahydrate, tablet 300 KAKKHKRHCKCAGH-UHFFFAOYSA-L 1S/C6H6NO6P.2Na.6H2O/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;;;;;;;/h1-4H,(H2,10,11,12);;;6*1H2/q;2*+1;;;;;;/p-2 SIGMA-ALDRICH - −20°C water, high purity: 0.25 tablet/mL, clear, colorless tablet
3578542 4-Nitrophenyl phosphate disodium salt hexahydrate, tablet 300 KAKKHKRHCKCAGH-UHFFFAOYSA-L 1S/C6H6NO6P.2Na.6H2O/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;;;;;;;/h1-4H,(H2,10,11,12);;;6*1H2/q;2*+1;;;;;;/p-2 SIGMA-ALDRICH - −20°C water, high purity: 0.25 tablet/mL, clear, colorless tablet
3578636 4-Nitrophenyl phosphate disodium salt hexahydrate, tablet 300 KAKKHKRHCKCAGH-UHFFFAOYSA-L 1S/C6H6NO6P.2Na.6H2O/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;;;;;;;/h1-4H,(H2,10,11,12);;;6*1H2/q;2*+1;;;;;;/p-2 SIGMA-ALDRICH 5 mg substrate per tablet −20°C - tablet
3578637 4-Nitrophenyl phosphate disodium salt hexahydrate, tablet 300 KAKKHKRHCKCAGH-UHFFFAOYSA-L 1S/C6H6NO6P.2Na.6H2O/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;;;;;;;/h1-4H,(H2,10,11,12);;;6*1H2/q;2*+1;;;;;;/p-2 SIGMA-ALDRICH 5 mg substrate per tablet −20°C - tablet
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