5-Bromo-4-chloro-3-indolyl beta-D-glucuronide sodium salt, >=98%
Application
Chromogenic substrate for β-glucuronidase (GUS) gene detection.
5-Bromo-4-chloro-3-indolyl ?-D-glucuronide sodium salt has been used:as a component in ?-glucuronidase (GUS) assay buffer for histochemical GUS assayto prepare X-Gluc or GUS staining solutionto confirm GUS expression in samples by X-gluc staining
Biochem/physiol Actions
5-bromo-4-chloro-3-indolyl-?-D-glucuronide sodium salt (X-GlcA), a colorless substrate for ?-glucuronidase enzyme, generates a blue color at the end of the reaction.
Packaging
10, 25, 100 mg in poly bottle
| Quality Level | 200 |
|---|---|
| InChI key | IBLSVGDGSKUDCT-CYRSAHDMSA-M |
| InChI | 1S/C14H13BrClNO7.Na/c15-4-1-2-5-7(8(4)16)6(3-17-5)23-14-11(20)9(18)10(19)12(24-14)13(21)22;/h1-3,9-12,14,17-20H,(H,21,22);/q;+1/p-1/t9-,10-,11+,12-,14+;/m0./s1 |
| Manufacturer | SIGMA-ALDRICH |
| Storage Temp. | −20°C |
| Solubility | water: 50 mg/mL, clear, colorless |
| Form | powder |
| Assay | ≥98% |
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