4-Nitrophenyl phosphate bis(cyclohexylammonium) salt, phosphatase substrate

Application

4-Nitrophenyl phosphate bis(cyclohexylammonium) salt has been used as a component in the colorimetric reagent for abscisic acid (ABA) assay.

Biochem/physiol Actions

p-Nitrophenyl phosphate (pNPP) is the preferred substrate for use with alkaline phosphatase in enzyme-linked immunosorbent assay (ELISA) procedures. Due to its high sensitivity, pNPP is the considered substrate in alkaline phosphatase systems. ELISA applications based on pNPP can be read in timed assays or delayed readings by stopping with alkaline solutions. This substrate yields a yellow-colored soluble end product that can be measured spectrophotometrically at 405 nm.

Packaging

1, 5, 25, 100 g in poly bottle

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Code Description Quality Level InChI key InChI Manufacturer Storage Temp. Solubility Form Assay Price
3578551 4-Nitrophenyl phosphate bis(cyclohexylammonium) salt, phosphatase substrate 300 KXNNUYCXODNFBD-UHFFFAOYSA-N 1S/C6H6NO6P.2C6H13N/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;2*7-6-4-2-1-3-5-6/h1-4H,(H2,10,11,12);2*6H,1-5,7H2 SIGMA-ALDRICH −20°C water: 10 mg/mL, clear, colorless to faintly yellow powder ≥97% anhydrous basis
3578552 4-Nitrophenyl phosphate bis(cyclohexylammonium) salt, phosphatase substrate 300 KXNNUYCXODNFBD-UHFFFAOYSA-N 1S/C6H6NO6P.2C6H13N/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;2*7-6-4-2-1-3-5-6/h1-4H,(H2,10,11,12);2*6H,1-5,7H2 SIGMA-ALDRICH −20°C water: 10 mg/mL, clear, colorless to faintly yellow powder ≥97% anhydrous basis
3578553 4-Nitrophenyl phosphate bis(cyclohexylammonium) salt, phosphatase substrate 300 KXNNUYCXODNFBD-UHFFFAOYSA-N 1S/C6H6NO6P.2C6H13N/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;2*7-6-4-2-1-3-5-6/h1-4H,(H2,10,11,12);2*6H,1-5,7H2 SIGMA-ALDRICH −20°C water: 10 mg/mL, clear, colorless to faintly yellow powder ≥97% anhydrous basis
3578554 4-Nitrophenyl phosphate bis(cyclohexylammonium) salt, phosphatase substrate 300 KXNNUYCXODNFBD-UHFFFAOYSA-N 1S/C6H6NO6P.2C6H13N/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;2*7-6-4-2-1-3-5-6/h1-4H,(H2,10,11,12);2*6H,1-5,7H2 SIGMA-ALDRICH −20°C water: 10 mg/mL, clear, colorless to faintly yellow powder ≥97% anhydrous basis
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