o-Dianisidine dihydrochloride, tablet, 10 mg substrate per tablet
Packaging
50, 100 tablets in glass bottle
Reconstitution
Dissolve one tablet in 60 ml of 50 mM phosphate-citrate buffer, pH 5.0. Add 12 µl of fresh 30% hydrogen peroxide immediately before to use.
Specificity
o-Dianisidine (3,3′-dimethoxybenzidine) is a peroxidase substrate suitable for use in ELISA procedures. This substrate produces a soluble end product that is yellow-orange in color and can be read spectrophotometrically at 405 nm. The reaction may be stopped with 5 M HCl.
Warning
Probably carcinogenic.
| Quality Level | 200 |
|---|---|
| InChI key | UXTIAFYTYOEQHV-UHFFFAOYSA-N |
| InChI | 1S/C14H16N2O2.2ClH/c1-17-13-7-9(3-5-11(13)15)10-4-6-12(16)14(8-10)18-2;;/h3-8H,15-16H2,1-2H3;2*1H |
| Manufacturer | SIGMA-ALDRICH |
| Storage Temp. | 2-8°C |
| Solubility | water: 1 tablet/10 mL, clear, colorless |
| Form | tablet |
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