N-Succinyl-L-phenylalanine-p-nitroanilide, protease substrate

Stock Code: 3578742
Manufacturer Part No: S2628-1G
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Application


N-Succinyl-L-phenylalanine-p-nitroanilide has been used as a substrate for the hydrolytic activity of chymotrypsin and molar absorptive experiments. It has also been used for monitoring in vitro ?-chymotrypsin inhibition assay.


Biochem/physiol Actions


N-Succinyl-L-phenylalanine-p-nitroanilide, a substrate for the hydrolytic activity of chymotrypsin, forms a yellow chromophore, p-nitroaniline, which can be measured spectrophotometrically at 410 nm.


Packaging


1, 5 g in poly bottle


Substrates


Reported to be a substrate for chymotrypsin.

Quality Level200
InChI keyKNBLWBFJHZHYFG-INIZCTEOSA-N
InChI1S/C19H19N3O6/c23-17(10-11-18(24)25)21-16(12-13-4-2-1-3-5-13)19(26)20-14-6-8-15(9-7-14)22(27)28/h1-9,16H,10-12H2,(H,20,26)(H,21,23)(H,24,25)/t16-/m0/s1
ManufacturerSIGMA-ALDRICH
Storage Temp.−20°C
Solubilitydioxane: 25 mg/mL, clear, colorless to faintly yellow
Formpowder
Assay≥98% (TLC)

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