L-Glutamic acid gamma-(p-nitroanilide) hydrochloride, gamma-glutamyl transpeptidase substrate

Stock Code: 3578318
Manufacturer Part No: G6133-5G

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Descriptions

Application

L-Glutamic acid ?-(p-nitroanilide) hydrochloride has been used as a solute carrier family 1 member 5 (SLC1A5) inhibitor:or an inhibitor of the cell membrane glutamine transporter to study the effects of blocking glutamine uptake on esophageal adenocarcinoma (EACC) and thioredoxin-interacting protein (TXNIP)in glutamine ELISA assay to treat confluent differentiated uninfected human colonoid monolayer (HCM) in apical and basolateral compartments to study its effectsto study its effect on SLC1A5_var-mediated mitochondrial glutamine transport inhibitionto study its effects on cellular glutathione levels, cellular reactive oxygen species (ROS) levels, and mitochondrial ROS levels

Biochem/physiol Actions

L-Glutamyl-p-nitroanilide (GPNA) is commonly used to block the glutamine (Gln) transporter alanine-serine-cysteine transporter 2 (ASCT2). It can also inhibit sodium-dependent and independent amino acid transporters. GPNA, ?-glutamyltransferase (GGT) substrate plays a key role in the hydrolysis of its ?-glutamyl bond and the subsequent release of the chromogen, p-nitroaniline (PNA).

Packaging

1, 5, 25 g in poly bottle

Substrates

Substrate for γ-glutamyl transpeptidase

Specifications

Quality Level	300
InChI key	OJEVFSFTVARWQX-UHFFFAOYSA-N
InChI	1S/C11H13N3O5.ClH/c12-9(11(16)17)5-6-10(15)13-7-1-3-8(4-2-7)14(18)19;/h1-4,9H,5-6,12H2,(H,13,15)(H,16,17);1H
Manufacturer	SIGMA-ALDRICH
Storage Temp.	2-8°C
Solubility	water: 5 mg/mL, clear, yellow
Form	powder
Assay	≥98% (HPLC)