Azocasein, protease substrate

Azocasein, protease substrate

Stock Code: 3578058
Manufacturer Part No: A2765-1G
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£60.45 (exc VAT) per 1G
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Application


Azocasein has been used as a substrate for determination of protease activity.


Azocasein is an inflammatory agent that is used to induce amyloid A amylooidosis in experimental animals.


Azocasein is a nonspecific protease substrate. Hydrolysis of the casein releases the azo dye into the media where it is detected by absorbance at 440 nm.


General description


Azocasein is a chromogenic derivative of casein. Protease degrades azocasein to yield TCA-soluble azopeptides with high UV-absorbance. This azocasein assay is widely employed to estimate the protease production by bacterial fermentation on synthetic substrates from glucose and inorganic salts.


Linkage


View more information on azocasein protease assay at www.sigma-aldrich.com/enzymeexplorer


Packaging


1, 5, 10, 25 g in glass bottle

Quality Level200
ManufacturerSIGMA-ALDRICH
Technique(s)ligand binding assay: suitable
Storage Temp.2-8°C
Solubilitywater: 5 mg/mL, clear, orange to very deep orange
Formpowder

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