7-Amino-4-methylcoumarin, Chromophore for substrates
Application
7-Amino-4-methylcoumarin has been used: to determine cathepsin B like- activity as a substrate for leucine aminopeptidase (LAP) in chymotryptic assayas a standard to detect the activation of caspase-3 during sanguinarine-induced damageprotection by human DEAD-box DDX3
Used as matrix for analysis of monosulfated disaccharides and as a co-matrix with 6-aza-2-thiothymidine for sulfated neutral and sialylated tri-and tetrasaccharides.
Biochem/physiol Actions
7-Amino-4-methylcoumarin is a coumarin derivative, which serves as a fluorescence reference standard to screen protease inhibitors.
Other Notes
Reagent for preparing new fluorogenic AMC-based substrates for cystine aminopeptidase (and other hydrolases); used as reference compound in the enzyme assay.
Packaging
1 g in poly bottle
250, 500 mg in poly bottle
Preparation Note
Soluble in DMSO (10 mg/mL), DMF, and acetone (10 mg/mL).
| Quality Level | 200 |
|---|---|
| mp | 223-226 °C (lit.) |
| InChI key | GLNDAGDHSLMOKX-UHFFFAOYSA-N |
| InChI | 1S/C10H9NO2/c1-6-4-10(12)13-9-5-7(11)2-3-8(6)9/h2-5H,11H2,1H3 |
| Manufacturer | SIGMA-ALDRICH |
| Further Info | chromophore for enzyme substrates |
| Form | powder |
| Assay | ≥98% (HPLC) |
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