7-Amino-4-methylcoumarin, Chromophore for substrates

Stock Code: 3578114
Manufacturer Part No: A9891-1G

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Descriptions

Application

7-Amino-4-methylcoumarin has been used: to determine cathepsin B like- activity as a substrate for leucine aminopeptidase (LAP) in chymotryptic assayas a standard to detect the activation of caspase-3 during sanguinarine-induced damageprotection by human DEAD-box DDX3

Used as matrix for analysis of monosulfated disaccharides and as a co-matrix with 6-aza-2-thiothymidine for sulfated neutral and sialylated tri-and tetrasaccharides.

Biochem/physiol Actions

7-Amino-4-methylcoumarin is a coumarin derivative, which serves as a fluorescence reference standard to screen protease inhibitors.

Other Notes

Reagent for preparing new fluorogenic AMC-based substrates for cystine aminopeptidase (and other hydrolases); used as reference compound in the enzyme assay.

Packaging

1 g in poly bottle

250, 500 mg in poly bottle

Preparation Note

Soluble in DMSO (10 mg/mL), DMF, and acetone (10 mg/mL).

Specifications

Quality Level	200
mp	223-226 °C (lit.)
InChI key	GLNDAGDHSLMOKX-UHFFFAOYSA-N
InChI	1S/C10H9NO2/c1-6-4-10(12)13-9-5-7(11)2-3-8(6)9/h2-5H,11H2,1H3
Manufacturer	SIGMA-ALDRICH
Further Info	chromophore for enzyme substrates
Form	powder
Assay	≥98% (HPLC)